Effect of <i>Astrocaryum aculeatum</i> (tucumã) on doxorubicin toxicity: <i>in vivo</i> experimental model

Carneiro, Alan Bruno Aurélio; Pinto, Eduardo Júnior Serrão; Ribeiro, Ivagner Ferreira; Magalhães, Mayck Rian Gonçalves; Monteiro, Moacir de Azevedo Bentes

doi:10.1590/1982-0194201700036

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Original Articles • Acta Paul Enferm. 30 (3) • May-Jun 2017 • https://doi.org/10.1590/1982-0194201700036 copy

Effect of Astrocaryum aculeatum (tucumã) on doxorubicin toxicity: in vivo experimental model

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Objective

To obtain the oil of Astrocaryum aculeatum (A.a), and evaluate its genotoxicity/antigenotoxicity activities using the micronucleus test in peripheral blood cells.

Methods

The oil of Astrocaryum aculeatum was obtained by hydraulic pressing. The animals used were healthy Swiss male mice, at 6-7 weeks of age; there were six per group. The genotoxic and antigenotoxic activity of concentrations were 500, 1,000 and 2,000 mg/kg per 0.5 mL (oral), followed or not followed by intraperitoneal injection of doxorubicin (0.3 mL-15 mg/kg by body weight), in addition to a negative group (water) and dimethyl sulfoxide (600 μL). Peripheral blood samples were collected 24h and 48h after treatment.

Results

A statistically significant reduction was identified in the frequency of micronuclei in polychromatic cells ranging from 34.72% to 38.19% for 24-hour treatments, and from 63.70% to 66.12% for 48 hour.

Conclusion

The fixed oil of tucumã presented antigenotoxic potential for the concentrations used in acute treatments.

Doxorubicin/toxicity; Plant extracts/analysis; Micronucleus tests

Treatment	Group	Dosage (mg/kg)
Negative control	1a,b	-
DMSO	2a	-
A. a I	3a	500
A. a II	4a	1,000
A. a III	5a	2,000
DXR	6a,b	15
DMSO + DXR	7b	As in (2) and (6)
A. a. I + DXR	8b	As in (3) and (6)
A. a II + DXR	9b	As in (4) and (6)
A. a III + DXR	10b	As in (5) and (6)

Treatment (mg/kg of body weight)	PCEMNs total		PCEMNs (mean ± SD)
Treatment (mg/kg of body weight)	24 h	48 h	24 h	48 h
Control	27	26	4.50 ± 0.55	4.33 ± 0.52
DMSO	31	34	5.16 ± 0.63	5.66 ± 0.82
A. a. I (500 mg)	29	29	5.16 ± 0.98	4.83 ± 0.41
A. a. II (1000 mg)	29	29	4.83 ± 0.75	4.83 ± 0.75
A. a. III (2000 mg)	28	28	4.66 ± 0.52	4.66 ± 0.52
DXR mg	171ª	274ª	28.50 ± 1.38	45.66 ± 1.63
DMSO + DXR	167ª	267ª	27.83 ± 0.75	44.66 ± 1.05
A. a. I + DXR	121ª^,b	116ª^,b	20.16 ± 1.16	19.33 ± 0.52
A. a. II + DXR	118ª^,b	111ª^,b	19.66 ± 1.03	18.50 ± 0.55
A. a. III + DXR	116ª^,b	110ª^,b	19.33 ± 1.37	18.33 ± 0.52

Treatment (mg/kg body weight)	IDN		PCEMNs/1,000 PCEs		REDUCTION (%)
Treatment (mg/kg body weight)	24 h	48 h	24 h	48 h	24 h	48 h
Control	0.011 ± 0.51	0.010 ± 0.51	2.25	2.16	-	-
DMSO	0.013 ± 0.54	0.011 ± 0.54	2.58	2.83	-	-
A. a. I	0.013 ± 0.54	0.011 ± 0.83	2.42	2.42	-	-
A. a. II	0.014 ± 0.75	0.013 ± 0.75	2.42	2.42	-	-
A. a. III	0.013 ± 0.83	0.013 ± 0.75	2.33	2.33	-	-
DXR	0.013 ± 1.03	0.012 ± 0.89	14.25	22.83	-	-
DMSO+DXR	0.013 ± 0.75	0.010 ± 0.51	14.08	22.25	-	-
A. a. I + DXR	0.012 ± 1.16	0.011 ± 0.81	11.33	9.66	34.72	63.70
A. a. II + DXR	0.013 ± 0.75	0.011 ± 0.83	10.08	9.25	36.80	65.72
A. a. III + DXR	0.012 ± 0.75	0.010 ± 0.51	9.66	9.16	38.19	66.12

Escola Paulista de Enfermagem, Universidade Federal de São Paulo R. Napoleão de Barros, 754, 04024-002 São Paulo - SP/Brasil, Tel./Fax: (55 11) 5576 4430 - São Paulo - SP - Brazil
E-mail: actapaulista@unifesp.br

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[1] Corresponding author. Alan Bruno Aurélio Carneiro. Rodovia Juscelino Kubitscheck, 68903-419, Macapá, AP, Brazil. abacarneiro@gmail.com