In vitro rooting was carried out on Murashige & Skoog (MS) and Wood Plant Medium (WPM) liquid and solid, suppiemented with indolbutiric acid (IBA). Multipleshoot induction was tested with benzylaminopurine (BAP) on MS and WPM Medium, with one explant in each tube. The explant was inoculated in container (200ml) on MS Medium suppiemented with BAP and NAA. The last experiment was made with BAP, GA3 and activaded charcoal. In the present work, the results show better rooting percentage and number of roots on WPM Medium with 4.9µM IBA. The multiplication of shoots was obtained with GA3, at 1.4µM that resulted in better lenght of shoots and number of buds. BAP was effective for all characteristics at 1.1µM only without actived charcoal.
micropropagation; growth regulator; tissue culture; Sebastiania schottiana
