Polygalacturonases enzyme was produced by solid state fermentation using as substrate the residue of passion fruit (Passiflora edulis flavicarpa) and as fermentation agent the mutant fungus Aspergillus niger CCT 0916. To optimize the recovery step of the enzymes, the values of the leaching parameters such as agitation, contact time and solvent/substrate relation were investigated with factorial experimental design 2³. Agitation of 50 rpm, 45 min and relation 10:1 provided the best recovery results. The response surface analysis, showed that the residue of passion fruit is an adequate substrate for the production of polygalacturonase enzyme.
recovery; extraction; Aspergillus niger; agro-industrial residue
